Protocols

Step-by-step workflows for running your own analysis, written like the protocols you already follow at the bench: what you need, what to set, and what to check before moving on.

RNA-seqBeginner5 steps

Bulk RNA-seq: from FASTQ to differentially expressed genes

Trim, quantify and test a two-condition experiment, then draw the PCA, volcano and heatmap to show it.

Single-cellIntermediate5 steps

Single-cell RNA-seq: from 10x reads to clusters and their markers

Count a 10x run, filter out empty droplets and dying cells, cluster what is left and find the genes that mark each cluster.

Follow these on your own data.

OpenBionode is in early access. Tell us what you analyse and we’ll make sure the protocol you need is ready.